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Cells were trypsinized for 5 min at 37 C, diluted in DMEM, and centrifuged at 400 g for 10 min

In contrast, enzyme activity following 4a incubation was not recovered on dilution, indicating an irreversible covalent bond with the enzyme (Supplementary Fig

Clin Cosmet Investig Dermatol

Injectable glutathione occupies a different space: it's an unapproved drug that physicians can still prescribe under the umbrella of medical practice and state compounding law, which means legality depends entirely on whether a prescriber is involved and whether the product is marketed as a drug

Carbohydr Polym (2008) 74:8404

While roGFP2 WT was completely oxidized at even very low PfGrx C32S/C88S (SSG) concentrations, 1 M PfGrx C32S/C88S (SSG) glutathionylated approximately 50% of 1 M roGFP2 C151S (SH) suggesting that the redox couples roGFP2 C151S (SSG)/roGFP2 C151S (SH) and PfGrx C32S/C88S (SSG)/PfGrx C32S/C88S (SH) have very similar redox potentials